Posted by: Indonesian Children | October 17, 2010

A cytologic assay for diagnosis of food hypersensitivity in patients with irritable bowel syndrome.

Clin Gastroenterol Hepatol. 2010 Mar;8(3):254-60. Epub 2009 Nov 22.

A cytologic assay for diagnosis of food hypersensitivity in patients with irritable bowel syndrome.

Carroccio A, Brusca I, Mansueto P, Pirrone G, Barrale M, Di Prima L, Ambrosiano G, Iacono G, Lospalluti ML, La Chiusa SM, Di Fede G.

Internal Medicine, Ospedali Civili Riuniti, Sciacca (Agrigento), Sciacca, Italy. acarroccio@hotmail.com

Abstract

BACKGROUND & AIMS: A percentage of patients with symptoms of irritable bowel syndrome (IBS) suffer from food hypersensitivity (FH) and improve on a food-elimination diet. No assays have satisfactory levels of sensitivity for identifying patients with FH. We evaluated the efficacy of an in vitro basophil activation assay in the diagnosis of FH in IBS-like patients.

METHODS: Blood samples were collected from 120 consecutive patients diagnosed with IBS according to Rome II criteria. We analyzed in vitro activation of basophils by food allergens (based on levels of CD63 expression), as well as total and food-specific immunoglobulin (Ig)E levels in serum. Effects of elimination diets and double-blind food challenges were used as standards for FH diagnosis.

RESULTS: Twenty-four of the patients (20%) had FH (cow’s milk and/or wheat hypersensitivity); their symptom scores improved significantly when they were placed on an elimination diet. Patients with FH differed from other IBS patients in that they had a longer duration of clinical history, a history of FH as children, and an increased frequency of self-reported FH; they also had hypersensitivities to other antigens (eg, egg or soy). The basophil activation assay diagnosed FH with 86% sensitivity, 88% specificity, and 87% accuracy; this level of sensitivity was significantly higher than that of serum total IgE or food-specific IgE assays.

CONCLUSIONS: A cytometric assay that quantifies basophils after stimulation with food antigens based on cell-surface expression of CD63 had high levels of sensitivity, specificity, and accuracy in diagnosing FH. This assay might be used to diagnose FH in patients with IBS-like symptoms.

Copyright 2010 AGA Institute. Published by Elsevier Inc. All rights reserved.

PMID: 19932763 [PubMed – indexed for MEDLINE]

Supported by

Clinic For Children Yudhasmara Foundation http://childrenclinic.wordpress.com/CHILDREN ALLERGY CLINIC https://childrenallergyclinic.wordpress.com/ 
PICKY EATERS CLINIC (KLINIK KESULITAN MAKAN) http://mypickyeaters.wordpress.com/ 
JL Taman Bendungan Asahan 5 Bendungan Hilir Jakarta Pusat Phone :62 (021) 70081995 – 5703646   

Clinical and Editor in Chief : Dr Widodo Judarwanto SpA, pediatrician

email : judarwanto@gmail.com,

 

 

 

 

 

                                                                                                            

Information on this web site is provided for informational purposes only and is not a substitute for professional medical advice. You should not use the information on this web site for diagnosing or treating a medical or health condition. You should carefully read all product packaging. If you have or suspect you have a medical problem, promptly contact your professional healthcare provider. 

Copyright © 2010, Clinic For Children Information Education Network. All rights reserved


Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

Categories

%d bloggers like this: